ITALY
Node Candidate Name: Advanced Light Microscopy Italian Node
The Italian ALM Node Candidate is a large multi-modal and multi-sited Node Candidate that comprises five imaging facilities located in Naples, Genoa, Padua, Florence and Milan. These facilities have a long experience of mutual interaction and collaboration. The Node Candidate is coordinated by the Institute of Protein Biochemistry, Naples, an institute belonging to the National Research Council of Italy. The Node Candidate provides open-access imaging services to both academia and industry and serves about 1000 users annually. While each location provides access to a wide range of technologies, they also specialize in one or two specific technologies as listed below. The Node Candidate offers a complete service package starting from sample preparation and imaging to final image analysis, quantitation and visualization.
Specialties and expertise of the Node Candidate
The facilities of this Node Candidate offer a broad range of services but they specialize in particular in the following technologies and research areas:
Technologies
Naples: Correlative light electron microscopy with 3D imaging, correlative microscopy with FRET imaging
Genoa: Super-resolution imaging
Padua: Functional imaging
Florence: Non-linear microscopy, functional imaging
Milan: Correlative microscopy with 3D tomography, elemental imaging, high-throughput microscopy
Research applications
Naples: Cell biology, membrane trafficking, signaling, cancer biology
Padua: Signaling, neurobiology
Milano: Neurobiology, cell biology, signaling, pharmacology
The member groups also have experience with a wide spectrum of model systems including mammalian cell culture systems, insect cell culture systems, and model organisms like Drosophila and mouse
Offered Technologies
Additional services offered at the Node Candidate
Instrument highlights
The members of the Italian ALM Node Candidate are also involved in the development of novel imaging methods, instrumentation and reagents. These include:
Naples: FRET-based methods to detect large multi-molecular complexes, Raman microscopy, image analysis methods and microfluidics
Genoa: various aspects of super-resolution microscopy
Padua: probes for functional imaging of intracellular ions and metabolites
Florence: functional imaging and Raman imaging
Milano: High-throughput microscopy
The Italian ALM Node Candidate is a large multi-modal and multi-sited Node Candidate that comprises five imaging facilities located in Naples, Genoa, Padua, Florence and Milan. These facilities have a long experience of mutual interaction and collaboration. The Node Candidate is coordinated by the Institute of Protein Biochemistry, Naples, an institute belonging to the National Research Council of Italy. The Node Candidate provides open-access imaging services to both academia and industry and serves about 1000 users annually. While each location provides access to a wide range of technologies, they also specialize in one or two specific technologies as listed below. The Node Candidate offers a complete service package starting from sample preparation and imaging to final image analysis, quantitation and visualization.
Specialties and expertise of the Node Candidate
The facilities of this Node Candidate offer a broad range of services but they specialize in particular in the following technologies and research areas:
Technologies
Naples: Correlative light electron microscopy with 3D imaging, correlative microscopy with FRET imaging
Genoa: Super-resolution imaging
Padua: Functional imaging
Florence: Non-linear microscopy, functional imaging
Milan: Correlative microscopy with 3D tomography, elemental imaging, high-throughput microscopy
Research applications
Naples: Cell biology, membrane trafficking, signaling, cancer biology
Padua: Signaling, neurobiology
Milano: Neurobiology, cell biology, signaling, pharmacology
The member groups also have experience with a wide spectrum of model systems including mammalian cell culture systems, insect cell culture systems, and model organisms like Drosophila and mouse
Offered Technologies
- Deconvolution widefield microscopy
- Laser scanning confocal microscopy (LSCM)
- Multiphoton systems
- Spinning disc confocal systems
- Total internal reflection fluorescence microscopy (TIRF)
- Fluorescence correlation spectroscopy (FCS)
- Fluorescence-lifetime imaging microscopy (FLIM)
- Fluorescence resonance energy transfer (FRET)
- Fluorescence recovery after photobleaching (FRAP)
- Raman Spectroscopy
- High Throughput Microscopy
- Electron microscopy
- Selective plane illumination microscopy (SPIM)
- 4Pi
- Ground state depletion microscopy (GSD)
- Photo activated localization microscopy (PALM)
- Stimulated emission depletion microscopy (STED)
- Stochastic optical reconstruction microscopy (STORM)
Additional services offered at the Node Candidate
- Support in experimental design
- Technical assistance to use the microscopes
- Practical training courses
- Cell culture facilities
- Wet lab space
- Animal facilities
- Data processing and analysis
Instrument highlights
The members of the Italian ALM Node Candidate are also involved in the development of novel imaging methods, instrumentation and reagents. These include:
Naples: FRET-based methods to detect large multi-molecular complexes, Raman microscopy, image analysis methods and microfluidics
Genoa: various aspects of super-resolution microscopy
Padua: probes for functional imaging of intracellular ions and metabolites
Florence: functional imaging and Raman imaging
Milano: High-throughput microscopy
Contact details
Alberto Luini
Coordinator of the Italian ALM Node Candidate
a.luini@ibp.cnr.it
+39 081 6132 535
Seetharaman Parashuraman
Head, Bioimaging facility, IBP-CNR
r.parashuraman@ibp.cnr.it
+39 081 6132283
Alberto Luini
Coordinator of the Italian ALM Node Candidate
a.luini@ibp.cnr.it
+39 081 6132 535
Seetharaman Parashuraman
Head, Bioimaging facility, IBP-CNR
r.parashuraman@ibp.cnr.it
+39 081 6132283