NETHERLANDS
Node Candidate Name: Facility of excellence in imaging - ALM and Molecular imaging Node Maastricht
The Facility of excellence in imaging - ALM and Molecular imaging Node Candidate in Maastricht is a state-of-the art molecular imaging Node Candidate that brings together a powerful palette of high-end and innovative molecular imaging technologies. The mission of this Node Candidate is to perform fundamental and applied studies in biological and molecular imaging as a part of a translational and interdisciplinary research program in a leading international research and educational center. The existing infrastructure contains a very complete set of both basic and high-end and sometimes unique imaging techniques, both on the microscopic and macroscopic level. The available microscopic techniques range from basic EM and optical microscopy to a range of state-of-the-art EM and optical microscopy, combined with an extensive palette of non-invasive imaging techniques.
Specialties and expertise of the Node Candidate
Our facility connects the newest and hottest in imaging instrumentation with societal relevant clinical and biomedical research areas. Specifically, we focus on cardiovascular research, due to our direct relation to the world-leading research school CARIM. However, also various other research areas are highly developed, due to the connection to the research schools NUTRIM (Metabolism), GROW (oncology and developmental biology), and MHeNS (brain). Additionally, due to extensive collaboration with the world leading school MERLN and the biochemical company DSM, we have strong interaction in the areas of biomaterials, catalysis, and biomass.
Offered Technologies
Additional services offered by the Node Candidate
Instrument highlights
With the broad range of techniques we offer, we have created an imaging platform, ranging from EM, via super-resolution STED, confocal, and multi-photon microscopy, towards whole body fluorescence imaging. This platform can easily be connected to non-invasive non-optical imaging methods, like MRI, PET, CT, and US.
The Facility of excellence in imaging - ALM and Molecular imaging Node Candidate in Maastricht is a state-of-the art molecular imaging Node Candidate that brings together a powerful palette of high-end and innovative molecular imaging technologies. The mission of this Node Candidate is to perform fundamental and applied studies in biological and molecular imaging as a part of a translational and interdisciplinary research program in a leading international research and educational center. The existing infrastructure contains a very complete set of both basic and high-end and sometimes unique imaging techniques, both on the microscopic and macroscopic level. The available microscopic techniques range from basic EM and optical microscopy to a range of state-of-the-art EM and optical microscopy, combined with an extensive palette of non-invasive imaging techniques.
Specialties and expertise of the Node Candidate
Our facility connects the newest and hottest in imaging instrumentation with societal relevant clinical and biomedical research areas. Specifically, we focus on cardiovascular research, due to our direct relation to the world-leading research school CARIM. However, also various other research areas are highly developed, due to the connection to the research schools NUTRIM (Metabolism), GROW (oncology and developmental biology), and MHeNS (brain). Additionally, due to extensive collaboration with the world leading school MERLN and the biochemical company DSM, we have strong interaction in the areas of biomaterials, catalysis, and biomass.
Offered Technologies
- Laser scanning confocal microscopy (LSCM)
- Multiphoton systems
- Spinning disc confocal systems
- Correlative light electron microscopy (CLEM)
- Fluorescence-lifetime imaging microscopy (FLIM)
- Fluorescence resonance energy transfer (FRET)
- Fluorescence recovery after photobleaching (FRAP)
- High Throughput Microscopy
- Electron microscopy
- Stimulated emission depletion microscopy (STED)
- High Field MRI
- MRI-PET
- microCT
- microMRI/MRS
- microSPECT/CT
- microUS
- Optical Imaging
Additional services offered by the Node Candidate
- Instruments
- Technical assistance to run instrument
- Methodological setup (e.g. design of study protocol and standard operation procedures)
- Training in infrastructure use
- Probe preparation
- Animal preparation
- Animal facilities
- Wet lab space
- Server Space
- Data processing and analysis
- Training workstations
- Training seminar room
Instrument highlights
With the broad range of techniques we offer, we have created an imaging platform, ranging from EM, via super-resolution STED, confocal, and multi-photon microscopy, towards whole body fluorescence imaging. This platform can easily be connected to non-invasive non-optical imaging methods, like MRI, PET, CT, and US.
3D rendered image of an ex vivo carotid artery, mounted on perfusion chamber (see reference) under pressure. The carotid artery underwent wire-injury, was mounted, followed by ex vivo injection of ultrasound microbubbles (Mbs) targeted to VCAM-1 (3 days after wire-injury). The MBs are rendered fluorescent by labeling them with rhodamine. Additional co-staining using antibody against PECAM-1 (green, endothelial cells) VCAM-1 (grey) and Syto41 (blue, cell nuclei) were performed. For more experimental details and the biological context see Curaj, A., et al., Noninvasive molecular ultrasound monitoring of vessel healing after intravascular surgical procedures in a preclinical setup. Arteriosclerosis, thrombosis, and vascular biology, 2015. 35(6): p. 1366.
Contact details
Marc A.M.J. van Zandvoort
Associate Professor Advanced Microscopic Imaging
mamj.vanzandvoort@maastrichtuniversity.nl
0031433881361
Marc A.M.J. van Zandvoort
Associate Professor Advanced Microscopic Imaging
mamj.vanzandvoort@maastrichtuniversity.nl
0031433881361
