FINLAND
Node Candidate Name: Finnish ALM Node - Advanced Light Microscopy Finnish Node
The Finnish advanced light microscopy (ALM) Node Candidate is based on three imaging facilities that have long experience in working together and offering open-access imaging services to both academia and industry, annually serving more than 850 users. The Node Candidate has expertise especially in 3D services across multiple platforms in light and electron microscopy, and is capable of providing advanced 3D imaging and 3D image visualization and analysis services also in areas where this is not always commonplace. The Node Candidate offers its services as full packages that include both image acquisition and bioimage informatics.
Specialties and expertise of the Node Candidate
The Finnish ALM Node Candidate focuses on three specialties that are state-of-the-art imaging technology areas: super-resolution imaging, correlative light and electron microscopy and mesoscopic imaging. The main research application areas covered in Finland with these technologies are metabolism and inflammation, developmental biology, cell migration, adhesion and invasion, quantitative cell biology, extracellular matrix biology, organotypic cultures, cardiovascular research, neurology and cancer biology. All of them covering a wide spectrum of model organisms and in vitro models.
Image analysis and bioinformatics are another area of expertise of the Finnish ALM Node Candidate and all three imaging facilities composing it engage in significant software development. The Node Candidate offers services through BioimageXD, an open-source platform for processing, visualization and analysis of images; the Center for Machine Vision Research with expertise in computer vision; and Microscopy Image Browser (MIB) for segmentation of multidimensional 3D electron microscopy data sets.
Offered Technologies
Additional services offered by the Node Candidate
Instrument highlights
Special features of instruments at the Finnish ALM Node Candidate are: 1) The combination of different imaging techniques such as correlative light and electron microscopy (CLEM) that combines electron microscopy with transmitted light or fluorescence microscopy. 2) The use of 3D imaging in super-resolution imaging techniques such as 3D tomographic STED (mirror-STED) and 3D in super-resolution localization techniques with N-STORM. 3) in-vivo mesoscopic scale multidimensional (time-lapse 3D) imaging using optical tomography (OPT) and light sheet microscopy (SPIM).
The Finnish advanced light microscopy (ALM) Node Candidate is based on three imaging facilities that have long experience in working together and offering open-access imaging services to both academia and industry, annually serving more than 850 users. The Node Candidate has expertise especially in 3D services across multiple platforms in light and electron microscopy, and is capable of providing advanced 3D imaging and 3D image visualization and analysis services also in areas where this is not always commonplace. The Node Candidate offers its services as full packages that include both image acquisition and bioimage informatics.
Specialties and expertise of the Node Candidate
The Finnish ALM Node Candidate focuses on three specialties that are state-of-the-art imaging technology areas: super-resolution imaging, correlative light and electron microscopy and mesoscopic imaging. The main research application areas covered in Finland with these technologies are metabolism and inflammation, developmental biology, cell migration, adhesion and invasion, quantitative cell biology, extracellular matrix biology, organotypic cultures, cardiovascular research, neurology and cancer biology. All of them covering a wide spectrum of model organisms and in vitro models.
Image analysis and bioinformatics are another area of expertise of the Finnish ALM Node Candidate and all three imaging facilities composing it engage in significant software development. The Node Candidate offers services through BioimageXD, an open-source platform for processing, visualization and analysis of images; the Center for Machine Vision Research with expertise in computer vision; and Microscopy Image Browser (MIB) for segmentation of multidimensional 3D electron microscopy data sets.
Offered Technologies
- Laser scanning confocal microscopy (LSCM/CLSM)
- Electron microscopy
- Multiphoton microscopy systems
- Photo activated localization microscopy (PALM)
- Spinning disc confocal microscopy (SDCM)
- Total internal reflection fluorescence microscopy (TIRF)
- Stimulated emission depletion microscopy (STED)
- Stochastic optical reconstruction microscopy (STORM)
- Correlative light electron microscopy (CLEM)
- Fluorescence correlation spectroscopy (FCS)
- Fluorescence cross-correlation spectroscopy (FCCS)
- Fluorescence-lifetime imaging microscopy (FLIM)
- Fluorescence resonance energy transfer (FRET)
- Fluorescence recovery after photobleaching (FRAP)
- Optical projection tomography (OPT)
- Selective plane illumination microscopy (SPIM)
Additional services offered by the Node Candidate
- Methodological setup (e.g. design of study protocol and standard operation procedures)
- Technical assistance to run instrument
- Animal facilities
- Wet lab space
- Data processing and analysis
- Training seminar room
- Cell culture facilities - Safety level 1 and 2
Instrument highlights
Special features of instruments at the Finnish ALM Node Candidate are: 1) The combination of different imaging techniques such as correlative light and electron microscopy (CLEM) that combines electron microscopy with transmitted light or fluorescence microscopy. 2) The use of 3D imaging in super-resolution imaging techniques such as 3D tomographic STED (mirror-STED) and 3D in super-resolution localization techniques with N-STORM. 3) in-vivo mesoscopic scale multidimensional (time-lapse 3D) imaging using optical tomography (OPT) and light sheet microscopy (SPIM).
Contact details
Gleb Grebnev
Finnish Advanced Light Microscopy Node Manager
Gleb.Grebnev@abo.fi
+358 (0)50 5724726
www.eurobioimaging.fi
Gleb Grebnev
Finnish Advanced Light Microscopy Node Manager
Gleb.Grebnev@abo.fi
+358 (0)50 5724726
www.eurobioimaging.fi