FLUORESCENCE-LIFETIME IMAGING MICROSCOPY (FLIM)
Fluorescence-lifetime imaging microscopy (FLIM) is an imaging technique for producing an image based on differences in the fluorescence-lifetime rather than its intensity. By quantifying variations in the exponential decay rate of the fluorescence from a fluorescent sample (fluorescence-lifetime) it is possible to report on molecule proximity, pH changes and even polarity. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy and multiphoton tomography. Since the fluorescence-lifetime is insensitive to changes in fluorophore intensity or concentration, it is the most quantitatively precise technique to report on fluoresce resonance energy transfer (FRET).
List of the Euro-BioImaging Node Candidates that offer fluorescence-lifetime imaging microscopy (FLIM) for interim operation :
- Czech Republic - ALM and Medical Imaging Node Brno CZ
- Czech Republic - Advanced Light And Electron Microscopy Node Prague CZ
- EMBL -Advanced Light Microscopy Facility EMBL
- Finland - Finnish ALM Node - Advanced Light Microscopy Finnish Node
- France - France BioImaging Node
- Italy - Advanced Light Microscopy Italian Node
- Netherlands - Facility of excellence in imaging - ALM and Molecular imaging Node Maastricht
- Netherlands - The Van Leeuwenhoek Center for Advanced Microscopy (LCAM) - Functional Imaging Flagship Node Amsterdam
- Netherlands - Wageningen Imaging and Spectroscopy Hub (WISH) - ALM and Molecular Imaging Node Wageningen
- Poland - Advanced Light Microscopy Polish Node
- Slovakia - Slovak BioImaging Network (SKBIN) - Functional Imaging Slovakian Flagship Node
- Spain - Barcelona Live and Intravital - Advanced Light Microscopy Node (BLivIN)
- Spain - Advanced Light Microscopy Node Bilbao Spain.