FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING (FRAP)
Fluorescence recovery after photobleaching (FRAP) denotes an optical technique capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes. This technique is very useful in biological studies of cell membrane diffusion and protein binding as it not only reports on the diffusion rates of mobile fractions of molecules but also provides information about the proportion of immobile molecules. In addition, surface deposition of a fluorescent phospholipid bilayer (or monolayer) allows the characterization of hydrophilic (or hydrophobic) surfaces in terms of surface structure and free energy.
List of the Euro-BioImaging Node Candidates that offer fluorescence recovery after photobleaching (FRAP) for interim operation :
- Belgium - Advanced Light Microscopy Belgian Node
- Czech Republic - Advanced Light And Electron Microscopy Node Prague CZ
- EMBL - Advanced Light Microscopy Facility EMBL
- Finland - Finnish ALM Node - Advanced Light Microscopy Finnish Node
- France - France BioImaging Node
- Italy - Advanced Light Microscopy Italian Node
- Netherlands - Facility of excellence in imaging - ALM and Molecular imaging Node Maastricht
- Netherlands - Erasmus MC OIC - Advanced Light Microscopy Rotterdam Node
- Netherlands - The Van Leeuwenhoek Center for Advanced Microscopy (LCAM) - Functional Imaging Flagship Node Amsterdam
- Netherlands - Wageningen Imaging and Spectroscopy Hub (WISH) - ALM and Molecular Imaging Node Wageningen
- Poland - Advanced Light Microscopy Polish Node
- Spain - Barcelona Live and Intravital - Advanced Light Microscopy Node (BLivIN)
- Spain - Advanced Light Microscopy Node Bilbao Spain.