FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING (FRAP)

 Fluorescence recovery after photobleaching (FRAP) denotes an optical technique capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes. This technique is very useful in biological studies of cell membrane diffusion and protein binding as it not only reports on the diffusion rates of mobile fractions of molecules but also provides information about the proportion of immobile molecules. In addition, surface deposition of a fluorescent phospholipid bilayer (or monolayer) allows the characterization of hydrophilic (or hydrophobic) surfaces in terms of surface structure and free energy.

List of the Euro-BioImaging Node Candidates that offer  fluorescence recovery after photobleaching (FRAP) for interim operation :