GROUND STATE DEPLETION MICROSCOPY (GSD) / GROUND STATE DEPLETION MICROSCOPY FOLLOWED BY INDIVIDUAL MOLECULE RETURN (GSDIM)
Ground state depletion microscopy followed by individual molecule return (GSDIM or GSD for short) is a super-resolution technique based on single molecule localization. To precisely localize single molecules and create a high-resolution image the ensemble of overlapping fluorophores are temporally "separated". This can be achieved by using high power lasers to transfer fluorophores into long-lived "off states" – a non-fluorescent molecule state. Single fluorophores return stochastically from the off state and emit bursts of photons, which are recorded. The position of the fluorophore is determined using a software algorithm. Based on this list of coordinates a super-resolution image is reconstructed.
List of the Euro-BioImaging Node Candidates that offer ground state depletion microscopy (GSD) / ground state depletion microscopy followed by individual molecule return (GSDIM) for interim operation :
- EMBL -Advanced Light Microscopy Facility EMBL
- France - France BioImaging Node
- Italy - Advanced Light Microscopy Italian Node
- Netherlands - The Van Leeuwenhoek Center for Advanced Microscopy (LCAM) - Functional Imaging Flagship Node Amsterdam
- Spain - SLN@BCN - Barcelona super Resolution Light Nanoscopy - Super Resolution Node Barcelona