GROUND STATE DEPLETION MICROSCOPY (GSD) / GROUND STATE DEPLETION MICROSCOPY FOLLOWED BY INDIVIDUAL MOLECULE RETURN (GSDIM)

Ground state depletion microscopy followed by individual molecule return (GSDIM or GSD for short) is a super-resolution technique based on single molecule localization. To precisely localize single molecules and create a high-resolution image the ensemble of overlapping fluorophores are temporally "separated". This can be achieved by using high power lasers to transfer fluorophores into long-lived "off states" – a non-fluorescent molecule state. Single fluorophores return stochastically from the off state and emit bursts of photons, which are recorded. The position of the fluorophore is determined using a software algorithm. Based on this list of coordinates a super-resolution image is reconstructed.

List of the Euro-BioImaging Node Candidates that offer ground state depletion microscopy (GSD) / ground state depletion microscopy followed by individual molecule return (GSDIM) for interim operation :

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