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GROUND STATE DEPLETION MICROSCOPY (GSD) / GROUND STATE DEPLETION MICROSCOPY FOLLOWED BY INDIVIDUAL MOLECULE RETURN (GSDIM)


Ground state depletion microscopy followed by individual molecule return (GSDIM or GSD for short) is a super-resolution technique based on single molecule localization. To precisely localize single molecules and create a high-resolution image the ensemble of overlapping fluorophores are temporally "separated". This can be achieved by using high power lasers to transfer fluorophores into long-lived "off states" – a non-fluorescent molecule state. Single fluorophores return stochastically from the off state and emit bursts of photons, which are recorded. The position of the fluorophore is determined using a software algorithm. Based on this list of coordinates a super-resolution image is reconstructed.

List of the Euro-BioImaging Node Candidates that offer ground state depletion microscopy (GSD) / ground state depletion microscopy followed by individual molecule return (GSDIM) for interim operation :


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  • EMBL -Advanced Light Microscopy Facility EMBL
  • France - France BioImaging Node
  • Italy - Advanced Light Microscopy Italian Node
  • Netherlands - The Van Leeuwenhoek Center for Advanced Microscopy (LCAM) - Functional Imaging Flagship Node Amsterdam
  • Spain - SLN@BCN - Barcelona super Resolution Light Nanoscopy - Super Resolution Node Barcelona 
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