OBJECTIVE-COUPLED PLANAR ILLUMINATION (OCPI)

An Objective-coupled planar illumination (OCPI) microscope is a microscope that rapidly sections a three-dimensional volume using a thin illumination sheet whose position is rigidly coupled to the objective and aligned with its focal plane. This method offers superior sensitivity and temporal resolution over laser-scanning microscopy for imaging rapid events in large populations of neurons. The reduced photobleaching associated with the method also allows optical recordings to be made over long periods of time.

List of the Euro-BioImaging Node Candidates that offer objective-coupled planar illumination (OCPI) for interim operation :