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PHOTO ACTIVATED LOCALIZATION MICROSCOPY (PALM)


Photo activated localization microscopy (PALM) is a widefield fluorescence microscopy imaging methods that allow obtaining images with a resolution beyond the diffraction limit. PALM is based on collecting a large number of images each containing just a few active isolated fluorophores. The imaging sequence allows for the many emission cycles necessary to stochastically activate each fluorophore from a non-emissive state to a bright state, and back to a bleached state. During each cycle, the density of activated molecules is kept low enough that the molecular images of individual fluorophores do not typically overlap.

List of the Euro-BioImaging Node Candidates that offer photo activated localization microscopy (PALM) for interim operation :


  • Belgium - Advanced Light Microscopy Belgian Node
  • Czech Republic - Advanced Light And Electron Microscopy Node Prague CZ
  • Finland - Advanced light microscopy Finnish node 
  • France - France BioImaging Node
  • Italy - Advanced Light Microscopy Italian Node
  • Netherlands - Erasmus MC OIC - Advanced Light Microscopy Rotterdam Node
  • Netherlands - The Van Leeuwenhoek Center for Advanced Microscopy (LCAM) - Functional Imaging Flagship Node Amsterdam
  • Poland - Advanced Light Microscopy Polish Node
  • Spain - SLN@BCN - Barcelona super Resolution Light Nanoscopy - Super Resolution Node Barcelona 
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