PHOTO ACTIVATED LOCALIZATION MICROSCOPY (PALM)
Photo activated localization microscopy (PALM) is a widefield fluorescence microscopy imaging methods that allow obtaining images with a resolution beyond the diffraction limit. PALM is based on collecting a large number of images each containing just a few active isolated fluorophores. The imaging sequence allows for the many emission cycles necessary to stochastically activate each fluorophore from a non-emissive state to a bright state, and back to a bleached state. During each cycle, the density of activated molecules is kept low enough that the molecular images of individual fluorophores do not typically overlap.
List of the Euro-BioImaging Node Candidates that offer photo activated localization microscopy (PALM) for interim operation :
- Belgium - Advanced Light Microscopy Belgian Node
- Czech Republic - Advanced Light And Electron Microscopy Node Prague CZ
- Finland - Advanced light microscopy Finnish node
- France - France BioImaging Node
- Italy - Advanced Light Microscopy Italian Node
- Netherlands - Erasmus MC OIC - Advanced Light Microscopy Rotterdam Node
- Netherlands - The Van Leeuwenhoek Center for Advanced Microscopy (LCAM) - Functional Imaging Flagship Node Amsterdam
- Poland - Advanced Light Microscopy Polish Node
- Spain - SLN@BCN - Barcelona super Resolution Light Nanoscopy - Super Resolution Node Barcelona