POLAND
Node Candidate Name: Advanced Light Microscopy Polish Node
The Polish Advanced Light Microscopy Node Candidate offers open access to imaging technologies and leading expertise as well as capacity/instrumentation in biological imaging including multi-modal ALM, CLEM, functional imaging, high-throughput microscopy, super-resolution microscopy. The ALM Polish Node Candidate provides full hands-on and theoretical training in all of the imaging techniques available at the Node Candidate facilities.
Specialties and expertise of the Node Candidate
Expertise in the study of life at all major levels of organization, beginning at the molecular and elemental levels, through subcellular organelle dynamics, cellular and tissue-level processes, as well as the whole organism. The main fields of expertise: single molecule detection, molecular interactions, protein dynamics, intracellular processes in neuronal cells, energy metabolism (in cancer, cardiovascular, neurodegeneration), cell biology (nucleus, mitochondria, cytoskeleton, neuromuscular junction, Golgi complex), physiology of the cell (intracellular membrane transport, membrane channels, apoptosis, regeneration stem cells, lymphoma cells, pharmacology).
Offered Technologies
Instrument highlights
Special features of instruments at the Polish ALM Node:
The Polish Advanced Light Microscopy Node Candidate offers open access to imaging technologies and leading expertise as well as capacity/instrumentation in biological imaging including multi-modal ALM, CLEM, functional imaging, high-throughput microscopy, super-resolution microscopy. The ALM Polish Node Candidate provides full hands-on and theoretical training in all of the imaging techniques available at the Node Candidate facilities.
Specialties and expertise of the Node Candidate
Expertise in the study of life at all major levels of organization, beginning at the molecular and elemental levels, through subcellular organelle dynamics, cellular and tissue-level processes, as well as the whole organism. The main fields of expertise: single molecule detection, molecular interactions, protein dynamics, intracellular processes in neuronal cells, energy metabolism (in cancer, cardiovascular, neurodegeneration), cell biology (nucleus, mitochondria, cytoskeleton, neuromuscular junction, Golgi complex), physiology of the cell (intracellular membrane transport, membrane channels, apoptosis, regeneration stem cells, lymphoma cells, pharmacology).
Offered Technologies
- Deconvolution widefield microscopy
- Laser scanning confocal microscopy (LSCM)
- Multiphoton systems
- Spinning disc confocal systems
- Total internal reflection fluorescence microscopy (TIRF)
- Fluorescence correlation spectroscopy (FCS)
- Fluorescence cross-correlation spectroscopy (FCCS)
- Fluorescence-lifetime imaging microscopy (FLIM)
- Fluorescence resonance energy transfer (FRET)
- Fluorescence recovery after photobleaching (FRAP)
- High Throughput Microscopy
- Electron microscopy
- Correlative light electron microscopy (CLEM)
- Photo activated localization microscopy (PALM)
- Stimulated emission depletion microscopy (STED)
Instrument highlights
Special features of instruments at the Polish ALM Node:
- Availability of array of different imaging modalities, including: spectral, two-photon, time-resolved and super-resolution fluorescence microscopy
- The use of 3D high-resolution imaging with block-face SEM tomography.
Contact details
Joanna Szczepanowska
Professor at Nencki IEB PAS
Jędrzej Szymański
eubi-node@nencki.gov.pl
+4822 5892345
Joanna Szczepanowska
Professor at Nencki IEB PAS
Jędrzej Szymański
eubi-node@nencki.gov.pl
+4822 5892345