REVERSIBLE SATURABLE OPTICAL FLUORESCENCE TRANSITION (RESOLFT)
Reversible saturable optical fluorescence transitions (RESOLFT) denotes a group of optical microscopy techniques with very high resolution. Using standard far field visible light optics a resolution far below the diffraction limit down to molecular scales can be obtained. STORM is based on collecting a large number of images each containing just a few active isolated fluorophores. Reversible switching between a fluorescent on-state and a dark off-state of a dye is exploited to acquire an imaging sequence where the position of a fluorophore is calculated with a precision typically greater than the diffraction limit. During each cycle, the density of activated molecules is kept low enough that the molecular images of individual fluorophores do not typically overlap.
List of the Euro-BioImaging Node Candidates that offer reversible saturable optical fluorescence transitions (RESOLFT) for interim operation :
- EMBL -Advanced Light Microscopy Facility EMBL
- France - France BioImaging Node