INTERIM WEB ACCESS PORTAL
  • Home
  • Locations and Technologies
  • Apply for access
  • Reporting and Feedback
  • Image Data Resource
  • Review of Applications
  • New Technologies
  • Document Gallery
  • BioImage Competition

REVERSIBLE SATURABLE OPTICAL FLUORESCENCE TRANSITION (RESOLFT)


Reversible saturable optical fluorescence transitions (RESOLFT) denotes a group of optical microscopy techniques with very high resolution. Using standard far field visible light optics a resolution far below the diffraction limit down to molecular scales can be obtained. STORM is based on collecting a large number of images each containing just a few active isolated fluorophores. Reversible switching between a fluorescent on-state and a dark off-state of a dye is exploited to acquire an imaging sequence where the position of a fluorophore is calculated with a precision typically greater than the diffraction limit. During each cycle, the density of activated molecules is kept low enough that the molecular images of individual fluorophores do not typically overlap.​ 

List of the Euro-BioImaging Node Candidates that offer reversible saturable optical fluorescence transitions (RESOLFT) for interim operation :


  • ​EMBL -Advanced Light Microscopy Facility EMBL
  • France - France BioImaging Node
Back
Disclaimer 
Video: EuBI access made easy
Contact
​
Feedback
www.eurobioimaging.eu
  • Home
  • Locations and Technologies
  • Apply for access
  • Reporting and Feedback
  • Image Data Resource
  • Review of Applications
  • New Technologies
  • Document Gallery
  • BioImage Competition