STOCHASTIC OPTICAL RECONSTRUCTION MICROSCOPY (STORM)
The fundamental principle behind stochastic optical reconstruction microscopy (STORM) is that the activated state of a photo-switchable molecule must lead to the consecutive emission of sufficient photons to enable precise localization before it enters a dark state or becomes deactivated by photobleaching. Additionally, the sparsely activated fluorescent molecules must be separated by a distance that exceeds the Abbe diffraction limit (in effect, greater than approximately 250 nanometers) to enable the parallel recording of many individual emitters, each having a distinct set of coordinates in the lateral image plane.
List of the Euro-BioImaging Node Candidates that offer stochastic optical reconstruction microscopy (STORM) for interim operation :
- Belgium - Advanced Light Microscopy Belgian Node
- Czech Republic - Advanced Light And Electron Microscopy Node Prague CZ
- EMBL -Advanced Light Microscopy Node EMBL
- Finland - Finnish ALM Node - Advanced Light Microscopy Finnish Node
- France - France BioImaging Node
- Hungary - Cellular Imaging Hungary
- Italy - Advanced Light Microscopy Italian Node
- Netherlands - Erasmus MC OIC - Advanced Light Microscopy Rotterdam Node
- Norway - NorMIC Oslo - Advanced Light Microscopy Node Oslo
- Spain - SLN@BCN - Barcelona super Resolution Light Nanoscopy - Super Resolution Node Barcelona