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STOCHASTIC OPTICAL RECONSTRUCTION MICROSCOPY (STORM)


The fundamental principle behind stochastic optical reconstruction microscopy (STORM)  is that the activated state of a photo-switchable molecule must lead to the consecutive emission of sufficient photons to enable precise localization before it enters a dark state or becomes deactivated by photobleaching. Additionally, the sparsely activated fluorescent molecules must be separated by a distance that exceeds the Abbe diffraction limit (in effect, greater than approximately 250 nanometers) to enable the parallel recording of many individual emitters, each having a distinct set of coordinates in the lateral image  plane. 

List of the Euro-BioImaging Node Candidates that offer stochastic optical reconstruction microscopy (STORM) for interim operation :


  • Belgium - Advanced Light Microscopy Belgian Node
  • Czech Republic - Advanced Light And Electron Microscopy Node Prague CZ
  • EMBL -Advanced Light Microscopy Node EMBL
  • Finland - Finnish ALM Node - Advanced Light Microscopy Finnish Node
  • France - France BioImaging Node
  • Hungary - Cellular Imaging Hungary
  • Italy - ​Advanced Light Microscopy Italian Node
  • Netherlands - Erasmus MC OIC - Advanced Light Microscopy Rotterdam Node
  • Norway - NorMIC Oslo - Advanced Light Microscopy Node Oslo​
  • Spain - SLN@BCN - Barcelona super Resolution Light Nanoscopy - Super Resolution Node Barcelona 
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